Recombinant humaan Nepirysine/CD10-eiwit

MME, also known as neprilysin, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. It degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. It is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL) . It is of use in hematological diagnosis since it is expressed by early B, pro-B and pre-B lymphocytes, and by lymph node germinal centers. Recombinant human MME, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

MME, Neprilysin, CALLA, CD10, CMT2T, NEP, SCA43, SFE, Atriopeptidase, Common acute lymphocyticleukemia antigen, Enkephalinase, Neutral endopeptidase, Skin fibroblast elastase, Membrane metalloendopeptidase, EPN

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

80.9 kDa (708aa)

Neprilysin, Neprilysin, MME, CALLA, CD10, CMT2T, NEP, SCA43, SFE, ATGP3646-10 µg, ATGP3646-20 µg, ATGP3646-50 µg, ATGP3646-100 µg, ATGP3646-250 µg, ATGP3646-500 µg, ATGP3646-1 mg, ATGP3646-10, ATGP3646-20, ATGP3646-50, ATGP3646-100, ATGP3646-250, ATGP3646-500, ATGP3646-1

Auer-Grumbach M., et al. (2016) Am J Hum Genet. 99:607-623.; ; Hama E., et al. (2005) Med Hypotheses. 65:498-500.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF, 10% glycerol

Cardiovascular

NP_000893.2

P08473

Human

Ala

YDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIP ETSSRYGNFD ILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLL KLLPDIYGWP VATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIH IDQPRLGLPS RDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVME LEKEIANATA KPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISI TNEEDVVVYA PEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKAL YGTTSETATW RRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDL TWMDAETKKR AEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFS QSKQLKKLRE KVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGI GMVIGHEITH GFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQH LNGINTLGEN IADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTY RPEYAVNSIK TDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VW