Recombinant humaan Nepirysine/CD10-eiwit

Neprilysin, also known as MME, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. It degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. It is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL) . It is of use in hematological diagnosis since it is expressed by early B, pro-B and pre-B lymphocytes, and by lymph node germinal centers. Recombinant human neprilysin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 5,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

MME, Neprilysin, CALLA, CD10, CMT2T, NEP, SCA43, SFE, Atriopeptidase, Common acute lymphocyticleukemia antigen, Enkephalinase, Neutral endopeptidase, Skin fibroblast elastase, Membrane metalloendopeptidase, EPN

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 5,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

80.9 kDa (708aa)

Neprilysin, ATGP3907-10 µg, ATGP3907-20 µg, ATGP3907-50 µg, ATGP3907-100 µg, ATGP3907-250 µg, ATGP3907-500 µg, ATGP3907-1 mg, ATGP3907-10, ATGP3907-20, ATGP3907-50, ATGP3907-100, ATGP3907-250, ATGP3907-500, ATGP3907-1

Auer-Grumbach M., et al. (2016) Am J Hum Genet. 99:607-623.; ; Hama E., et al. (2005) Med Hypotheses. 65:498-500.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF, 10% glycerol

Immunology

NP_000893

P08473

Human

Ala

YDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIPETSSRYGNFDILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLLKLLPDIYGWPVATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIHIDQPRLGLPSRDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVMELEKEIANATAKPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISITNEEDVVVYAPEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKALYGTTSETATWRRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDLTWMDAETKKRAEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFSQSKQLKKLREKVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGIGMVIGHEITHGFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQHLNGINTLGENIADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTYRPEYAVNSIKTDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VW