Recombinant humaan NEU-1/Sialidase-1-eiwit

NEu1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEu1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sialidase 1 (lysosomal sialidase), NANH, NEu, SIAL1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

42.9 kDa (393aa) confirmed by MALDI-TOF

NEU1, Sialidase1, Sialidase 1 (lysosomal sialidase), NANH, NEu, SIAL1, ATGP1685-10 µg, ATGP1685-20 µg, ATGP1685-50 µg, ATGP1685-100 µg, ATGP1685-250 µg, ATGP1685-500 µg, ATGP1685-1 mg, ATGP1685-10, ATGP1685-20, ATGP1685-50, ATGP1685-100, ATGP1685-250, ATGP1685-500, ATGP1685-1

Abdulkhalek, S., et al. (2011) J. Biol. Chem. 286 (42), 36532-36549

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_000425.1

Q99519

Human

ENDFG LVQPLVTMEQ LLWVSGRQIG SVDTFRIPLI TATPRGTLLA FAEARKMSSS DEGAKFIALR RSMDQGSTWS PTAFIVNDGD VPDGLNLGAV VSDVETGVVF LFYSLCAHKA GCQVASTMLV WSKDDGVSWS TPRNLSLDIG TEVFAPGPGS GIQKQREPRK GRLIVCGHGT LERDGVFCLL SDDHGASWRY GSGVSGIPYG QPKQENDFNP DECQPYELPD GSVVINARNQ NNYHCHCRIV LRSYDACDTL RPRDVTFDPE LVDPVVAAGA VVTSSGIVFF SNPAHPEFRV NLTLRWSFSN GTSWRKETVQ LWPGPSGYSS LATLEGSMDG EEQAPQLYVL YEKGRNHYTE SISVAKISVY GTL