Recombinant humaan NEURL2-eiwit

NEuRL2 plays an important role in the process of myofiber differentiation and maturation. This protein is probable substrate-recognition component of a SCF-like ECS (Elongin BC-CuL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination of proteins. It probably contributes to catalysis through recognition and positioning of the substrate and the ubiquitin-conjugating enzyme. During myogenesis, NEuRL2 controls the ubiquitination and degradation of the specific pool of CTNNB1/beta-catenin located at the sarcolemma. Recombinant human NEuRL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neuralized homolog 2, C20orf163, OZZ, Ozz-E3, Neuralized E3 ubiquitin protein ligase 2, Neuralized-like 2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.1 kDa (308aa)

Neuralized homolog 2, C20orf163, OZZ, Ozz-E3, Neuralized E3 ubiquitin protein ligase 2, Neuralized-like 2, NEuRL2, Ozz E3, Neuralized like 2, ATGP2297-10 µg, ATGP2297-20 µg, ATGP2297-50 µg, ATGP2297-100 µg, ATGP2297-250 µg, ATGP2297-500 µg, ATGP2297-1 mg, ATGP2297-010, ATGP2297-020, ATGP2297-050, ATGP2297-100, ATGP2297-250, ATGP2297-500, ATGP2297-01M

Aurino, S., et al. (2008) Acta Myol 27, 90-97; ; Song, R., et al. (2006) J. Biol. Chem. 281 (47), 36391-36400

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol

Ubiquitination

NP_542787

Q9BR09

Human

MAAASEP VDSGALWGLE RPEPPPTRFH RVHGANIRVD PSGTRATRVE SFAHGVCFSR EPLAPGQVFL VEIEEKELGW CGHLRLGLTA LDPASLAPVP EFSLPDLVNL GHTWVFAITR HHNRVPREGR PEAEAAAPSR PPTLLVEPYL RIEQFRIPRD RLVGRSRPGL YSHLLDQLYE LNVLPPTARR SRLGVLFCPR PDGTADMHII INGEDMGPSA RGLPAAQPLY AVVDVFASTK SVRLVQLEYG LPSLQTLCRL VIQRSMVHRL AIDGLHLPKE LKDFCKYE