Recombinant humaan Neurogranin eiwit

NRGN, also known as Neurogranin, is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. This protein is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. Recombinant human NRGN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Neurogranin, NRGN, HNG, RC3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

10 kDa (101aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Neurogranin, NRGN, HNG, RC3, ATGP2734-10 µg, ATGP2734-20 µg, ATGP2734-50 µg, ATGP2734-100 µg, ATGP2734-250 µg, ATGP2734-500 µg, ATGP2734-1 mg, ATGP2734-010, ATGP2734-020, ATGP2734-050, ATGP2734-100, ATGP2734-250, ATGP2734-500, ATGP2734-01M

Martinez de Arrieta, C. et al. (1999) Endocrinology. 140: 335-343; ; Broadbelt K. et al. (2006) Schizophr Res. 87:6-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH7.0) containing 30% glycerol 0.1mM PMSF,1mM EDTA

Neuroscience

NP_001119653

Q92686

Human

MGSSHHHHHH SSGLVPRGSH MGSMDCCTEN ACSKPDDDIL DIPLDDPGAN AAAAKIQASF RGHMARKKIK SGERGRKGPG PGGPGGAGVA RGGAGGGPSG D