Recombinant humaan Neuropiline 1/NRP1-eiwit

NRP1, also known as neuropilin-1 isoform a, is a type 1 transmembrane protein. This protein is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor and semaphorin family members. It is a VEGF165 and semaphorin receptor expressed by vascular endothelial cells (EC) and tumor cells. It is a co-receptor for VEGF receptor (VEGFR2) that enhances the binding of VEGF 165 to VEGFR2 and VEGF165-mediated chemotaxis. Also, it plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. Recombinant human NRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Neuropilin-1 isoform a, NRP1, BDCA4, CD304, NP1, NRP, VEGF165R, Vascular endothelial cell growth factor 165 receptor

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

94.8 kDa (843aa)

NRP1, ATGP3769-10 µg, ATGP3769-20 µg, ATGP3769-50 µg, ATGP3769-100 µg, ATGP3769-250 µg, ATGP3769-500 µg, ATGP3769-1 mg, ATGP3769-10, ATGP3769-20, ATGP3769-50, ATGP3769-100, ATGP3769-250, ATGP3769-500, ATGP3769-1

Soker S., et al, (1998) Cell 92:735-745.; ; He Z., et al, (1997) Cell 90:739-751.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Angiogenesis

NP_003864.4

O14786

Human

Phe22