Recombinant humaan NFATC2-eiwit

NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Nuclear factor of activated T-cells cytoplasmic 2 isoform C, NFATC2, NFAT1, NFATP

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

33.1 kDa (290aa)

NFATC2, Nuclear factor of activated T-cells cytoplasmic 2 isoform C, NFATC2, NFAT1, NFATP, ATGP3772-10 µg, ATGP3772-20 µg, ATGP3772-50 µg, ATGP3772-100 µg, ATGP3772-250 µg, ATGP3772-500 µg, ATGP3772-1 mg, ATGP3772-10, ATGP3772-20, ATGP3772-50, ATGP3772-100, ATGP3772-250, ATGP3772-500, ATGP3772-1

Gabriel CH., et al, (2016) J. Biol. Chem. 291:24172-24187.; ; Ho S., et al, (1994) Adv. Exp. Med. Biol. 365:167-173.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol, 1mM DTT

Signal Transduction

NP_775114

Q13469

Human

Pro396