Recombinant humaan NFU1-eiwit

NFU1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFU1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NFU1 iron-sulfur cluster scaffold, HIRA interacting protein 5, NFU1 iron-sulfur cluster scaffold homolog, HIRIP5, CGI-33, NifU, NIFUC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.9 kDa (268aa) confirmed by MALDI-TOF

NFu1, NFu1 iron-sulfur cluster scaffold homolog mitochondrial, NFu1 iron-sulfur cluster scaffold homolog, mitochondrial, CGI-33, HIRIP, HIRIP5, MMDS1, Nfu, Nifu, NIFuC, ATGP2888-10 µg, ATGP2888-20 µg, ATGP2888-50 µg, ATGP2888-100 µg, ATGP2888-250 µg, ATGP2888-500 µg, ATGP2888-1 mg, ATGP2888-10, ATGP2888-20, ATGP2888-50, ATGP2888-100, ATGP2888-250, ATGP2888-500, ATGP2888-1

Ganesh S., et al (2003) . Hum. Mol. Genet. 12:2359-2368; ; Tong W.-H., et al (2003) . Proc. Natl. Acad. Sci. u.S.A. 100:9762-9767;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Other

NP_001002755.1

Q9UMS0

Human

GAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPV RYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENE ELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKG FEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP