Recombinant humaan NHEJ1-eiwit

Non-homologous end-joining factor 1, also known as NHEJ1, belongs to the XLF family. NHEJ1 was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with NHEJ1 mutations also have immunodeficiency due to a defect in V (D) J recombination, which utilizes NHEJ to promote immune system diversity. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the ligation step of NHEJ. Recombinant human NHEJ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Non-homologous end-joining factor 1, XLF

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.8 kDa (247aa) confirmed by MALDI-TOF

NHEJ1, Non-homologous end-joining factor 1, XLF, ATGP1684-10 µg, ATGP1684-20 µg, ATGP1684-50 µg, ATGP1684-100 µg, ATGP1684-250 µg, ATGP1684-500 µg, ATGP1684-1 mg, ATGP1684-10, ATGP1684-20, ATGP1684-50, ATGP1684-100, ATGP1684-250, ATGP1684-500, ATGP1684-1

Buck D., et al. (2006) Cell. 124:287-299; ; Ahnesorg P., et al. (2006) Cell. 124:301-313

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Epigenomics (Transcription & Translation)

NP_079058

Q9H9Q4

Human

MGSSHHHHHH SSGLVPRGSH MGSMEELEQG LLMQPWAWLQ LAENSLLAKV FITKQGYALL VSDLQQVWHE QVDTSVVSQR AKELNKRLTA PPAAFLCHLD NLLRPLLKDA AHPSEATFSC DCVADALILR VRSELSGLPF YWNFHCMLAS PSLVSQHLIR PLMGMSLALQ CQVRELATLL HMKDLEIQDY QESGATLIRD RLKTEPFEEN SFLEQFMIEK LPEACSIGDG KPFVMNLQDL YMAVTTQ