Recombinant humaan NHLH1-eiwit

The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. NHLH1 may serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system. Recombinant human NHLH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nescient helix loop helix 1, bHLHa35, HEN1, NSCL, NSCL1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

17 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NHLH1, Nescient helix loop helix 1, bHLHa35, HEN1, NSCL, NSCL1, ATGP2200-10 µg, ATGP2200-20 µg, ATGP2200-50 µg, ATGP2200-100 µg, ATGP2200-250 µg, ATGP2200-500 µg, ATGP2200-1 mg, ATGP2200-10, ATGP2200-20, ATGP2200-50, ATGP2200-100, ATGP2200-250, ATGP2200-500, ATGP2200-1

Brown L., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:8492-8496; ; Lipkowitz S., et al. (1992) J. Biol. Chem. 267:21065-21071;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_005589

Q02575

Human

MMLNSDT MELDLPPTHS ETESGFSDCG GGAGPDGAGP GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR VEAFNLAFAE LRKLLPTLPP DKKLSKIEIL RLAICYISYL NHVLDV