Recombinant humaan NHP2-eiwit

NHP2, also called NOLA2, forms a small ribonucleoprotein particle with GAR1 (NOLA1), Nop10 for the isomerization of uridine to pseudouridine. This protein is involved in various aspects of rRNA processing and modification. It localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies. Recombinant human NHP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

H/ACA ribonucleoprotein complex subunit 2 isoform a, NHP2P, NOLA2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.3 kDa (173aa) confirmed by MALDI-TOF

NHP2, H/ACA ribonucleoprotein complex subunit 2 isoform a, NHP2P, NOLA2, ATGP0865-10 µg, ATGP0865-20 µg, ATGP0865-50 µg, ATGP0865-100 µg, ATGP0865-250 µg, ATGP0865-500 µg, ATGP0865-1 mg, ATGP0865-10, ATGP0865-20, ATGP0865-50, ATGP0865-100, ATGP0865-250, ATGP0865-500, ATGP0865-1

Henras A., et al. (1998), EMBO J., 17 (23) :7078-90.; ; Pogacic V., et al. (2000) . Mol. Cell. Biol.: 20:9028-9040;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_060308

Q9NX24

Human

MGSSHHHHHH SSGLVPRGSH MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL