Recombinant humaan NHP2L1-eiwit

NHP2L1 belongings to the ribosomal protein L7Ae family. This protein localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. It directly binds to the 5' stem-loop of u4 snRNA and plays an important role in the late stage of spliceosome assembly, prior to step I of splicing catalysis. Recombinant human NHP2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

NHP2 (non-histone chromosome protein 2) -like 1, FA1, NHPX, OTK27, SNRNP15-5, SNu13, SPAG12, SSFA1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.7 kDa (152aa) confirmed by MALDI-TOF

NHP2L1, NHP2 (non-histone chromosome protein 2) -like 1, FA1, NHPX, OTK27, SNRNP15-5, SNu13, SPAG12, SSFA1, ATGP0812-10 µg, ATGP0812-20 µg, ATGP0812-50 µg, ATGP0812-100 µg, ATGP0812-250 µg, ATGP0812-500 µg, ATGP0812-1 mg, ATGP0812-10, ATGP0812-20, ATGP0812-50, ATGP0812-100, ATGP0812-250, ATGP0812-500, ATGP0812-1

Luhrmann R., et al. (2006) Mol Cell Biol. 26 (13) :5146-54.; ; Jovin TM., et al (2005) RNA. 11 (2) :197-209.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol

Signal Transduction

NP_004999

P55769

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV