Recombinant humaan Nicotinamide N-methyltransferase/NNMT-eiwit

NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Nicotinamide N-methyltransferase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.7 kDa (284aa) confirmed by MALDI-TOF

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase, NNMT, Nicotinamide N-methyltransferase EC 2.1.1.1 Nicotinamide N methyltransferase., ATGP0320-10 µg, ATGP0320-20 µg, ATGP0320-50 µg, ATGP0320-100 µg, ATGP0320-250 µg, ATGP0320-500 µg, ATGP0320-1 mg, ATGP0320-10, ATGP0320-20, ATGP0320-50, ATGP0320-100, ATGP0320-250, ATGP0320-500, ATGP0320-1

Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Enzymes & Proteases

NP_006160

P40261

Human

MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL