Recombinant humaan Nicotinamide N-methyltransferase/NNMT-eiwit

NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Nicotinamide N-methyltransferase

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

37.7 kDa (284aa) confirmed by MALDI-TOF

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase, ATGP3681-10 µg, ATGP3681-20 µg, ATGP3681-50 µg, ATGP3681-100 µg, ATGP3681-250 µg, ATGP3681-500 µg, ATGP3681-1 mg, ATGP3681-10, ATGP3681-20, ATGP3681-50, ATGP3681-100, ATGP3681-250, ATGP3681-500, ATGP3681-1

Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Enzymes & Proteases

NP_006160

P40261

Human

MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL