Recombinant humaan niereiwit

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release, vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. Recombinant human REN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Renin, HNFJ2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

39.9 kDa (365aa)

Renin, Renin, HNFJ2, ATGP2177-10 µg, ATGP2177-20 µg, ATGP2177-50 µg, ATGP2177-100 µg, ATGP2177-250 µg, ATGP2177-500 µg, ATGP2177-1 mg, ATGP2177-10, ATGP2177-20, ATGP2177-50, ATGP2177-100, ATGP2177-250, ATGP2177-500, ATGP2177-1

Gribouval O., et al. (2005) Nat. Genet. 37:964-968; ; Zivna M., et al. (2009) Am. J. Hum. Genet. 85:204-213

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_000528

P00797

Human

LTLGN TTSSVILTNY MDTQYYGEIG IGTPPQTFKV VFDTGSSNVW VPSSKCSRLY TACVYHKLFD ASDSSSYKHN GTELTLRYST GTVSGFLSQD IITVGGITVT QMFGEVTEMP ALPFMLAEFD GVVGMGFIEQ AIGRVTPIFD NIISQGVLKE DVFSFYYNRD SENSQSLGGQ IVLGGSDPQH YEGNFHYINL IKTGVWQIQM KGVSVGSSTL LCEDGCLALV DTGASYISGS TSSIEKLMEA LGAKKRLFDY VVKCNEGPTL PDISFHLGGK EYTLTSADYV FQESYSSKKL CTLAIHAMDI PPPTGPTWAL GATFIRKFYT EFDRRNNRIG FALAR