Recombinant humaan NIP7-eiwit

NIP7, also known as the 60S ribosome subunit biogenesis protein NIP7 homolog, belongs to NIP7 family and contains 1 PuA domain. It interacts with pre-ribosome complex and may bind to RNA. This protein is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly. Recombinant human NIP7 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

60S ribosome subunit biogenesis protein NIP7 homolog, CGI 37, FLJ10296, HSPC031, HSPC180, KD93, NIP 7, Nuclear import 7, Nuclear import 7 homolog (S. cerevisiae), OK/SW cl.76, OK/SW cl.78

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.5 kDa (188aa) confirmed by MALDI-TOF

60S ribosome subunit biogenesis protein NIP7 homolog, CGI 37, CGI37, CGI-37, FLJ10296, HSPC031, HSPC180, KD93, NIP 7, NIP7, Nuclear import 7, Nuclear import 7 homolog (S. cerevisiae), OK/SW cl.76, OK/SW cl.78, OK/SW cl.78., ATGP0545-10 µg, ATGP0545-20 µg, ATGP0545-50 µg, ATGP0545-100 µg, ATGP0545-250 µg, ATGP0545-500 µg, ATGP0545-1 mg, ATGP0545-010, ATGP0545-020, ATGP0545-050, ATGP0545-100, ATGP0545-250, ATGP0545-500, ATGP0545-01M

Sekiguchi T., et al. (2004) J. Biol. Chem. 279 (9) :8343-50.; ; Hesling C., et al. (2007) Exp. Cell. Res. 313 (20) :4180-95.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_057185

Q9Y221

Human