Recombinant humaan NIT2-eiwit

Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein. Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Omega-amidase NIT2, Nitrilase homolog 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33 kDa (299aa) confirmed by MALDI-TOF

NIT2, Omega-amidase NIT2, Nitrilase homolog 2, ATGP0819-10 µg, ATGP0819-20 µg, ATGP0819-50 µg, ATGP0819-100 µg, ATGP0819-250 µg, ATGP0819-500 µg, ATGP0819-1 mg, ATGP0819-10, ATGP0819-20, ATGP0819-50, ATGP0819-100, ATGP0819-250, ATGP0819-500, ATGP0819-1

Barqliw K T., et al. (2008) Biochemistry. 47 (51) :13514-23.; ; Camarqo A., et al. (2007) Plant Cell. 19 (11) :3491-503.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_064587

Q9NQR4

Human

MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP