Recombinant humaan NKIRAS1-eiwit

NKIRAS1, also known as NF-kappa-B inhibitor-interacting Ras-like protein 1, belongs to the small GTPase superfamily. NKIRAS1 is atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. It may act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Recombinant human NKIRAS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

NF-kappa-B inhibitor-interacting Ras-like protein 1, kappaB-Ras1, KBRAS1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.8 kDa (212aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NKIRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, kappaB-Ras1, KBRAS1, ATGP1412-10 µg, ATGP1412-20 µg, ATGP1412-50 µg, ATGP1412-100 µg, ATGP1412-250 µg, ATGP1412-500 µg, ATGP1412-1 mg, ATGP1412-10, ATGP1412-20, ATGP1412-50, ATGP1412-100, ATGP1412-250, ATGP1412-500, ATGP1412-1

Fenwick C., et al. (2000) Science. 287:869-873; ; Chen Y., et al. (2003) J. Biol. Chem. 278:23101-23106

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_065078.1

Q9NYS0

Human

MGKGCKVVVC GLLSVGKTAI LEQLLYGNHT IGMEDCETME DVYMASVETD RGVKEQLHLY DTRGLQEGVE LPKHYFSFAD GFVLVYSVNN LESFQRVELL KKEIDKFKDK KEVAIVVLGN KIDLSEQRQV DAEVAQQWAK SEKVRLWEVT VTDRKTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNS EN