Recombinant humaan NM23-H2/NME2-eiwit

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

NME/NM23 nucleoside diphosphate kinase 2, Nucleoside diphosphate kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, NDK B, NDP kinase B, NM23B, NDPKB

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.2 kDa (152aa) confirmed by MALDI-TOF

NM23-H2, NME2, Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B, Nucleoside diphosphate kinase B C myc purine binding transcription factor PuF, NDK B, NDP kinase B, NM23 H2, Non-Metastatic Cells 2, MGC111212, ATGP0490-10 µg, ATGP0490-20 µg, ATGP0490-50 µg, ATGP0490-100 µg, ATGP0490-250 µg, ATGP0490-500 µg, ATGP0490-1 mg, ATGP0490-10, ATGP0490-20, ATGP0490-50, ATGP0490-100, ATGP0490-250, ATGP0490-500, ATGP0490-1

Munkonge FM., et al. (2009) J Biol Chem. 284 (39) :26978-87.; ; Treharne KJ., et al. (2009) FEBS Lett. 583 (17) :2789-92.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001018149.1

P22392

Human

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE