Recombinant humaan NM23-H2/NME2-eiwit

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

NME/NM23 nucleoside diphosphate kinase 2, Nucleoside diphosphate kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, NDK B, NDP kinase B, NM23B, NDPKB

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

17.2 kDa (152aa) confirmed by MALDI-TOF

NME2, Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B, ATGP3251-10 µg, ATGP3251-20 µg, ATGP3251-50 µg, ATGP3251-100 µg, ATGP3251-250 µg, ATGP3251-500 µg, ATGP3251-1 mg, ATGP3251-10, ATGP3251-20, ATGP3251-50, ATGP3251-100, ATGP3251-250, ATGP3251-500, ATGP3251-1

Munkonge FM., et al. (2009) J Biol Chem. 284 (39) :26978-87.; ; Treharne KJ., et al. (2009) FEBS Lett. 583 (17) :2789-92.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001018149

P22392

Human

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE