Recombinant humaan NME3-eiwit

NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3, Non-Metastatic Cells 3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19.1 kDa (169aa) confirmed by MALDI-TOF

NME3, Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3, Non-Metastatic Cells 3, ATGP0889-10 µg, ATGP0889-20 µg, ATGP0889-50 µg, ATGP0889-100 µg, ATGP0889-250 µg, ATGP0889-500 µg, ATGP0889-1 mg, ATGP0889-10, ATGP0889-20, ATGP0889-50, ATGP0889-100, ATGP0889-250, ATGP0889-500, ATGP0889-1

Martinez R, et al. (1997) . Cancer Res. 57: 1180-1187.; ; Masse K., et al. (2002) . Gene., 296: 87-97

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol 0.1M NaCl, 2mM DTT

Metabolism

NP_002504

Q13232

Human

ERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE