Recombinant humaan NMI-eiwit

N-myc-interactor, also known as NMI, is a member of the oncogene Myc family. The Myc family of oncogenes is thought to play an important role in cell proliferation, differentiation, and neoplastic transformation. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. Recombinant human NMI protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

N-myc-interactor

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.2 kDa (327aa) confirmed by MALDI-TOF

NMI, N-myc-interactor, ATGP0874-10 µg, ATGP0874-20 µg, ATGP0874-50 µg, ATGP0874-100 µg, ATGP0874-250 µg, ATGP0874-500 µg, ATGP0874-1 mg, ATGP0874-10, ATGP0874-20, ATGP0874-50, ATGP0874-100, ATGP0874-250, ATGP0874-500, ATGP0874-1

Bao J., et al. (1996) Oncogene. 16 (10) :217-6.; ; Bannasch D., et al. (1999) Oncogene. 18:6810-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Cancer

NP_004679

Q13287

Human

MEADKDDTQQ ILKEHSPDEF IKDEQNKGLI DEITKKNIQLK KEIQKLETE LQEATKEFQI KEDIPETKMK FLSVETPEND SQLSNISCSF QVSSKVPYEI QKGQALITFE KEEVAQNVVS MSKHHVQIKDV NLEVTAKPV PLNSGVRFQV YVEVSKMKIN VTEIPDTLRE DQMRDKLELS FSKSRNGGGE VDRVDYDRQS GSAVITFVEI GVADKILKKKEYPLYINQTC HRVTVSPYTE IHLKKYQIFS GTSKRTVLLT GMEGIQMDEE IVEDLINIHF QRAKNGGGEV DVVKCSLGQP HIAYFEE