Recombinant humaan NMNAT-1-eiwit

NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, PNAT1, NMNAT

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 7,000pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

36.0 kDa (315aa) confirmed by MALDI-TOF

PNAT1, PNAT 1, NMNAT1, NMNAT 1, NMNAT, NMN/NaMN adenylyltransferase1, NMN/NaMN adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase1, Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, ATGP3194-10 µg, ATGP3194-20 µg, ATGP3194-50 µg, ATGP3194-100 µg, ATGP3194-250 µg, ATGP3194-500 µg, ATGP3194-1 mg, ATGP3194-010, ATGP3194-020, ATGP3194-050, ATGP3194-100, ATGP3194-250, ATGP3194-500, ATGP3194-01M

Emanuelli M. et al. (2001) J Biol Chem. 276 (1) :406-12.; ; Zhou T, ., et al. (2002) J Biol Chem. 277 (15) :13148-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT 1mM EDTA

Neuroscience

NP_073624

Q9HAN9

Human

MENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK