Recombinant humaan NMRAL1-eiwit

NMRAL1 is a redox sensor protein that undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. Recombinant human NMRAL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NmrA-like family domain containing 1, HSCARG, SDR48A1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.9 kDa (323aa) confirmed by MALDI-TOF

NMRAL1, NmrA-like family domain containing 1, HSCARG, SDR48A1, ATGP1586-10 µg, ATGP1586-20 µg, ATGP1586-50 µg, ATGP1586-100 µg, ATGP1586-250 µg, ATGP1586-500 µg, ATGP1586-1 mg, ATGP1586-10, ATGP1586-20, ATGP1586-50, ATGP1586-100, ATGP1586-250, ATGP1586-500, ATGP1586-1

Gan.Q., et al. (2009) J. Cell. Sci. 122 (PT 22), 4081-4088; ; Lian, M., et al. (2009) J. Biol. Chem. 284 (27), 17998-18006

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl

Metabolism

NP_065728

Q9HBL8

Human

MVDKKL VVVFGGTGAQ GGSVARTLLE DGTFKVRVVT RNPRKKAAKE LRLQGAEVVQ GDQDDQVIME LALNGAYATF IVTNYWESCS QEQEVKQGKL LADLARRLGL HYVVYSGLEN IKKLTAGRLA AAHFDGKGEV EEYFRDIGVP MTSVRLPCYF ENLLSHFLPQ KAPDGKSYLL SLPTGDVPMD GMSVSDLGPV VLSLLKMPEK YVGQNIGLST CRHTAEEYAA LLTKHTRKVV HDAKMTPEDY EKLGFPGARD LANMFRFYAL RPDRDIELTL RLNPKALTLD QWLEQHKGDF NLL