Recombinant humaan NMT1-eiwit

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

59.2 kDa (519aa)

NMT1, Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1, ATGP2932-10 µg, ATGP2932-20 µg, ATGP2932-50 µg, ATGP2932-100 µg, ATGP2932-250 µg, ATGP2932-500 µg, ATGP2932-1 mg, ATGP2932-10, ATGP2932-20, ATGP2932-50, ATGP2932-100, ATGP2932-250, ATGP2932-500, ATGP2932-1

Glover C.J. et al. (1997) J. Biol. Chem. 272:28680-28689.; ; Van Damme P. et al. (2012) Proc. Natl. Acad. Sci. u.S.A. 109:12449-12454.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Cancer

NP_066565.1

P30419

Human

MADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ