Recombinant humaan NOB1-eiwit

In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5. 8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. Recombinant human NOB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

RNA-binding protein NOB1, RNA-binding protein NOB1, ART-4, MST158, MSTP158, NOB1P, PSMD8BP1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

49.1 kDa (435aa)

NOB1, RNA-binding protein NOB1, RNA-binding protein NOB1, ART-4, MST158, MSTP158, NOB1P, PSMD8BP1, ATGP2331-10 µg, ATGP2331-20 µg, ATGP2331-50 µg, ATGP2331-100 µg, ATGP2331-250 µg, ATGP2331-500 µg, ATGP2331-1 mg, ATGP2331-10, ATGP2331-20, ATGP2331-50, ATGP2331-100, ATGP2331-250, ATGP2331-500, ATGP2331-1

Zhang Y., et al. (2005) Kidney Int. 79:1138-1148; ; Daub H., et al. (2008) Mol. Cell. 31:438-448;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_054781

Q9ULX3

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WITPSNIKQI QQELEQCDVP EDVRVGCLTT DFAMQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPRGLRYSLP TPKGGKYAIN PHLTEDQRFP QLRLSQKARQ KTNVFAPDYI AGVSPFVEND ISSRSATLQV RDSTLGAGRR RLNPNASRKK FVKKR