Recombinant humaan norrin-eiwit

Norrin precursor, also known as NDP, is a secreted regulatory protein. It activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. This protein plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. It may be involved in a pathway that regulates neural cell differentiation and proliferation. Recombinant human NDP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Norrin, EVR2, FEVR, ND, NDP

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

14.8 kDa (132aa)

Norrin, Norrin, EVR2, FEVR, ND, ATGP2290-10 µg, ATGP2290-20 µg, ATGP2290-50 µg, ATGP2290-100 µg, ATGP2290-250 µg, ATGP2290-500 µg, ATGP2290-1 mg, ATGP2290-10, ATGP2290-20, ATGP2290-50, ATGP2290-100, ATGP2290-250, ATGP2290-500, ATGP2290-1

Luhmann u F., et al. (2005) Invest Pothalmol Vis Sci. 46:3372-3382.; ; Luhmann u F., et al. (2005) Genesis. 42:253-262

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Neuroscience

NP_000257.1

Q00604

Human

KTDSSFI MDSDPRRCMR HHYVDSISHP LYKCSSKMVL LARCEGHCSQ ASRSEPLVSF STVLKQPFRS SCHCCRPQTS KLKALRLRCS GGMRLTATYR YILSCHCEEC NS