Recombinant humaan NPL-eiwit

NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism. Recombinant human NPL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

N-acetylneuraminate lyase, c112, C1orf13, DHDPS1, MGC149582, MGC61869, NPL1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.3 kDa (340aa) confirmed by MALDI-TOF

NPL, N-acetylneuraminate lyase, c112, C1orf13, DHDPS1, MGC149582, MGC61869, NPL1, ATGP1028-10 µg, ATGP1028-20 µg, ATGP1028-50 µg, ATGP1028-100 µg, ATGP1028-250 µg, ATGP1028-500 µg, ATGP1028-1 mg, ATGP1028-10, ATGP1028-20, ATGP1028-50, ATGP1028-100, ATGP1028-250, ATGP1028-500, ATGP1028-1

Schauer R. et al. (1982) Adv Carbohydr Chem Biochem. 40:131-234.; ; COMB DG. et al. (1960) J Biol Chem. 235:2529-2537

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_110396

Q9BXD5

Human

MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEW VTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLD GILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQ RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS