Recombinant humaan NPM1-eiwit

NPM1 is a phosphoprotein which moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in NPM1 are associated with acute myeloid leukemia. Recombinant human NPM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleophosmin, B23, NPM

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.7 kDa (314aa) confirmed by MALDI-TOF

NPM1, ATGP1576-10 µg, ATGP1576-20 µg, ATGP1576-50 µg, ATGP1576-100 µg, ATGP1576-250 µg, ATGP1576-500 µg, ATGP1576-1 mg, ATGP1576-10, ATGP1576-20, ATGP1576-50, ATGP1576-100, ATGP1576-250, ATGP1576-500, ATGP1576-1

Sagawa, F., et al. (2011) EMBO J. 30 (19), 3994-4005.; ; Neubauer, A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

Nakatomi K, et al. TLR4/MD-2 is a receptor for extracellular nucleophosmin 1. (Biomed Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/33335727/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl,1mM DTT

Epigenomics (Transcription & Translation)

NP_002511.1

P06748

Human

MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK VPQKKVKLAA DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL