Recombinant humaan NPM2-eiwit

NPM2, also known as nucleophosmin/nucleoplasmin 2, belongs to the nucleoplasmin family. This protein is core histones chaperone involved in chromatin reprogramming, especially during fertilization and early embryonic development. It probably involved in sperm DNA decondensation during fertilization. Recombinant human NPM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleophosmin/ nucleoplasmin 2, NPM-2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.6 kDa (237aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NPM2, nucleophosmin/ nucleoplasmin 2, NPM-2, ATGP1676-10 µg, ATGP1676-20 µg, ATGP1676-50 µg, ATGP1676-100 µg, ATGP1676-250 µg, ATGP1676-500 µg, ATGP1676-1 mg, ATGP1676-10, ATGP1676-20, ATGP1676-50, ATGP1676-100, ATGP1676-250, ATGP1676-500, ATGP1676-1

Platonova O., et al. (2011) Biochemistry 50:8078-8089

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

Development

NP_877724

Q86SE8

Human

MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK