Recombinant humaan NQO1-eiwit

NQO1 is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD) . Recombinant human NQO1, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,000 pmol/min/ug and is defined as the amount of enzyme that converts 1 pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

NAD (P) H quinone dehydrogenase 1, NMOR1, DIA4, Diaphorase, NADH/NADPH, Cytochrome b-5 reductase, NAD (P) H dehydrogenase, Quinone 1, DHQU, QR1, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD (P) H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug and is defined as the amount of enzyme that converts 1pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

33.0 kDa (294aa) confirmed by MALDI-TOF

NQO1, ATGP3876-10 µg, ATGP3876-20 µg, ATGP3876-50 µg, ATGP3876-100 µg, ATGP3876-250 µg, ATGP3876-500 µg, ATGP3876-1 mg, ATGP3876-10, ATGP3876-20, ATGP3876-50, ATGP3876-100, ATGP3876-250, ATGP3876-500, ATGP3876-1

Jaiswal AK., et al. (1988) J Biol Chem. 263 (27) :13572-8.; ; Traver RD., et al. (1992) Cancer Res. 52 (4) :797-802.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Redox Proteins

NP_000894

P15559

Human

MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK