Recombinant humaan NQO2-eiwit

NQO2 is a member of the NAD (P) H dehydrogenase (quinone) . The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. Recombinant human NQO2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

N-ribosyldihydronicotinamide:quinone reductase 2, NMOR2, NAD (P) H menadione oxidoreductase 2, Dioxin-inducible, NAD (P) H quinone dehydrogenase 2, Quinone reductase 2, QR2, DHQV, DIA6, NRH:quinone oxidoreductase 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.1 kDa (251aa) confirmed by MALDI-TOF

NQO2, Ribosyldihydronicotinamide dehydrogenase, DHQV, DIA6, QR2, Ribosyldihydronicotinamide dehydrogenase Ox2, EC 1.10.99.2, MGC94180, NAD (P) H dehydrogenase quinone 2, NAD (P) H menadione oxidoreductase 1 dioxin inducible 2, NAD (P) H menadione oxidoreductase 2 dioxin inducible, NMOR2, NQO 2, NRH dehydrogenase, NRH:quinone oxidoreductase 2, OTTHuMP00000015948, OTTHuMP00000015949, OTTHuMP00000015953, Ox 2, QR2, Quinone, Quinone reductase 2, Ribosyldihydronicotinamide dehydrogenase., ATGP0522-10 µg, ATGP0522-20 µg, ATGP0522-50 µg, ATGP0522-100 µg, ATGP0522-250 µg, ATGP0522-500 µg, ATGP0522-1 mg, ATGP0522-10, ATGP0522-20, ATGP0522-50, ATGP0522-100, ATGP0522-250, ATGP0522-500, ATGP0522-1

Wu K., et al. (1997) Arch Biochem Biophys. 347 (2) :221-8. ; ; Jaiswal AK., et al. (1994) J Biol Chem. 269 (20) :14502-8. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Redox Proteins

AAH06096

P16083

Human

MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNFEPR ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q