Recombinant humaan NRG1/HRG1-eiwit

Neuregulin-1 (NRG1), also known as ARIA, is one of four proteins in the neuregulin family that act on the EGFRfamily of receptors. It has multiple isoforms that aregenerated by usage of different promoters and alternative splicing of a single gene. This protein regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination. In the heart, it regulates organ morphogenesis and contractility and also plays a cardioprotective role following tissue injury. NRG1 is essential for the development and function of multiple organ systems, and itsdysregulation has been linked to diseases such as cancer and schizophrenia. Recombinant human NRG1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatographytechniques.

Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 15 ng/mL.

NRG1, ARIA, GGF2, GGF, GGFglial growth factor, HGL, HGLneu differentiation factor, HRG, HRG1, HRGA, NRG-IT2, NRG1-IT2, HRG1-alpha, HRG1-beta 1, HRGAneuregulin 1 type IV beta 1a, Heregulin-1, MST131, MSTP131, NDFheregulin, alpha (45kD, ERBB2 p185-activator), Neuregulin-1, Neuregulin1, SMDF, neuregulin 1, neuregulin 1 type IV beta 3, pro-NRG1, pro-neuregulin-1, membrane-bound isoform, sensory and motor neuron derived factor

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 15 ng/mL.

25.1kDa (231aa)

, ATGP3990-10 µg, ATGP3990-20 µg, ATGP3990-50 µg, ATGP3990-100 µg, ATGP3990-250 µg, ATGP3990-500 µg, ATGP3990-1 mg, ATGP3990-10, ATGP3990-20, ATGP3990-50, ATGP3990-100, ATGP3990-250, ATGP3990-500, ATGP3990-1

Tan W, et al. (2007) . J Biol Chem. 282:24343-24351.; ; Nicodemus KK, et al. (2010) . Arch Gen Psychiatry. 67:991-1001.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_039252

Q02297

Human

Ala

SGKKPES AAGSQSPALP PRLKEMKSQE SAAGSKLVLR CETSSEYSSL RFKWFKNGNE LNRKNKPQNI KIQKKPGKSE LRINKASLAD SGEYMCKVIS KLGNDSASAN ITIVESNEII TGMPASTEGA YVSSESPIRI SVSTEGANTS SSTSTSTTGT SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYSTSTPF LSLPE