Recombinant humaan NSDHL-eiwit

NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Sterol-4-alpha-carboxylate 3-dehydrogenase decarboxylating, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, H105E3, SDR31E1, XAP104

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

35.5 kDa (320aa) confirmed by MALDI-TOF

NSDHL, Sterol-4-alpha-carboxylate 3-dehydrogenase decarboxylating, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, H105E3, SDR31E1, XAP104, ATGP2460-10 µg, ATGP2460-20 µg, ATGP2460-50 µg, ATGP2460-100 µg, ATGP2460-250 µg, ATGP2460-500 µg, ATGP2460-1 mg, ATGP2460-10, ATGP2460-20, ATGP2460-50, ATGP2460-100, ATGP2460-250, ATGP2460-500, ATGP2460-1

Morimoto M. et al. (2012) J Mol Histol. 43:95-106; ; McLarren KW. et al. (2010) Am J Hum Genet 87:905-914.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Metabolism

NP_057006

Q15738

Human

MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY