Recombinant humaan NSMCE1-eiwit

NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) . NSMCE1 has in vitro ubiquitin ligase activity in presence of NDNL2. Recombinant human NSMCE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Non-structural maintenance of chromosomes element 1 homolog, NSE1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33 kDa (289aa) confirmed by MALDI-TOF

NSMCE1, Non-structural maintenance of chromosomes element 1 homolog, NSE1, ATGP2231-10 µg, ATGP2231-20 µg, ATGP2231-50 µg, ATGP2231-100 µg, ATGP2231-250 µg, ATGP2231-500 µg, ATGP2231-1 mg, ATGP2231-10, ATGP2231-20, ATGP2231-50, ATGP2231-100, ATGP2231-250, ATGP2231-500, ATGP2231-1

Hudson JJ, Bednarova K, et al. (2011) . PLoS One. 6 (2) :e17270.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_659547

Q8WV22

Human

MQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GIRMHLPCVA KYFQSNAEPR CPHCNDYWPH EIPKVFDPEK ERESGVLKSN KKSLRSRQH