Recombinant humaan NT5C2-eiwit

NT5C2, also known as NT5B or PNT5, has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis. Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Cytosolic purine 5'-nucleotidase, cN-II, GMP. NT5B, PNT5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

67.1 kDa (581aa)

NT5C2, Cytosolic purine 5'-nucleotidase, cN-II, GMP. NT5B, PNT5, ATGP1229-10 µg, ATGP1229-20 µg, ATGP1229-50 µg, ATGP1229-100 µg, ATGP1229-250 µg, ATGP1229-500 µg, ATGP1229-1 mg, ATGP1229-10, ATGP1229-20, ATGP1229-50, ATGP1229-100, ATGP1229-250, ATGP1229-500, ATGP1229-1

Oka J. et al. (1994) Biochem. Biophys. Res. Commun. 205: 917-922.; ; Wallde K. et al. (2007) J Biol Chem. 282: 17828-17836.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Enzymes & Proteases

NP_036361.1

P49902

Human

MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E