Recombinant humaan NT5C3B-eiwit

NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m (7) GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m (7) GMP may protect cells against undesired salvage of m (7) GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

7-methylguanosine phosphate-specific 5'-nucleotidase, NT5C3L

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.8 kDa (323aa) confirmed by MALDI-TOF

NT5C3B, 7-methylguanosine phosphate-specific 5'-nucleotidase, NT5C3L, ATGP2887-10 µg, ATGP2887-20 µg, ATGP2887-50 µg, ATGP2887-100 µg, ATGP2887-250 µg, ATGP2887-500 µg, ATGP2887-1 mg, ATGP2887-10, ATGP2887-20, ATGP2887-50, ATGP2887-100, ATGP2887-250, ATGP2887-500, ATGP2887-1

Brieger A. et al. (2010) Proteomics. 10:3343-3355.; ; Aksoy P. et al. (2009) Pharmacogenet Genomics. 19:567-576.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_443167

Q969T7

Human

MAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP