Recombinant humaan NT5M eiwit

NT5M, also known as DNT2, belongs to the 5' (3') -deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2' (3') -phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11. 2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant human NT5M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

5' (3') -deoxyribonucleotidase mitochondrial, 5' (3') -deoxyribonucleotidase, mitochondrial, DNT2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.1 kDa (218aa) confirmed by MALDI-TOF

NT5M, 5' (3') -deoxyribonucleotidase mitochondrial, 5' (3') -deoxyribonucleotidase, mitochondrial, DNT2, ATGP1164-10 µg, ATGP1164-20 µg, ATGP1164-50 µg, ATGP1164-100 µg, ATGP1164-250 µg, ATGP1164-500 µg, ATGP1164-1 mg, ATGP1164-10, ATGP1164-20, ATGP1164-50, ATGP1164-100, ATGP1164-250, ATGP1164-500, ATGP1164-1

Rampazzo C., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8239-8244; ; Rinaldo-Matthis A., et al. (2002) Nat. Struct. Biol. 9:779-787

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Metabolism

NP_064586.1

Q9NPB1

Human

GGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC