Recombinant humaan NTH1-eiwit

NTHL1, also known as nth endonuclease III-like protein 1, is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines. Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Endonuclease III-like protein 1, NTH1, OCTS3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

36.6 kDa (332aa) confirmed by MALDI-TOF

NTH1, Endonuclease III-like protein 1, NTH1, OCTS3, ATGP1217-10 µg, ATGP1217-20 µg, ATGP1217-50 µg, ATGP1217-100 µg, ATGP1217-250 µg, ATGP1217-500 µg, ATGP1217-1 mg, ATGP1217-10, ATGP1217-20, ATGP1217-50, ATGP1217-100, ATGP1217-250, ATGP1217-500, ATGP1217-1

Imai K., et al. (1998) Gene. 222:287-295; ; Ikeda S., et al. (1998) J. Biol. Chem. 273:21585-21593

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Enzymes & Proteases

NP_002519.1

P78549

Human

MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL