Recombinant humaan NUBP1-eiwit

NUBP1 also known as cytosolic Fe-S cluster assembly factor NUBP1 isoform 1. NUBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NUBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytosolic NUBP iron-sulfur cluster assembly factor 1, Cytosolic Fe-S cluster assembly factor NUBP1, Nucleotide-binding protein 1, NBP1, NBP35, CIAO5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.9 kDa (343aa) confirmed by MALDI-TOF

NuBP1, Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, NBP, NBP1, NBP35, ATGP3046-10 µg, ATGP3046-20 µg, ATGP3046-50 µg, ATGP3046-100 µg, ATGP3046-250 µg, ATGP3046-500 µg, ATGP3046-1 mg, ATGP3046-10, ATGP3046-20, ATGP3046-50, ATGP3046-100, ATGP3046-250, ATGP3046-500, ATGP3046-1

Stehling O., et al. (2008) Mol. Cell. Biol. 28:5517-5528.; ; Bienvenut W.V., et al. (2012) Mol. Cell. Proteomics 11:M111.015131-M111.015131;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_002475.2

P53384

Human

MEEVPHD CPGADSAQAG RGASCQGCPN QRLCASGAGA TPDTAIEEIK EKMKTVKHKI LVLSGKGGVG KSTFSAHLAH GLAEDENTQI ALLDIDICGP SIPKIMGLEG EQVHQSGSGW SPVYVEDNLG VMSVGFLLSS PDDAVIWRGP KKNGMIKQFL RDVDWGEVDY LIVDTPPGTS DEHLSVVRYL ATAHIDGAVI ITTPQEVSLQ DVRKEINFCR KVKLPIIGVV ENMSGFICPK CKKESQIFPP TTGGAELMCQ DLEVPLLGRV PLDPLIGKNC DKGQSFFIDA PDSPATLAYR SIIQRIQEFC NLHQSKEENL ISS