Recombinant humaan NUBP2-eiwit

NuBP2 is an adenosine triphosphate (ATP) and metal-binding protein that is required for the assembly of cyotosolic iron-sulfur proteins. This protein functions in a heterotetramer with nucleotide-binding protein 1 (NuBP1) . Alternative splicing results in multiple transcript variants. Recombinant human NuBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytosolic Fe-S cluster assembly factor NUBP2, Nucleotide-binding protein 2, NBP 2, CFD1, CIAO6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.2 kDa (294aa) confirmed by MALDI-TOF

NuBP2, Cytosolic Fe-S cluster assembly factor NuBP2, Cytosolic Fe-S cluster assembly factor NuBP2, Nucleotide binding protein 2, CFD1, NBP 2, NuBP1, ATGP2895-10 µg, ATGP2895-20 µg, ATGP2895-50 µg, ATGP2895-100 µg, ATGP2895-250 µg, ATGP2895-500 µg, ATGP2895-1 mg, ATGP2895-10, ATGP2895-20, ATGP2895-50, ATGP2895-100, ATGP2895-250, ATGP2895-500, ATGP2895-1

Nakashima H., et al. (1999) Genomics. 60 (2) : 152-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 1mM DTT

Metabolism

NP_036357

Q9Y5Y2

Human

MGSSHHHHHH SSGLVPRGSH MGSMEAAAEP GNLAGVRHII LVLSGKGGVG KSTISTELAL ALRHAGKKVG ILDVDLCGPS IPRMLGAQGR AVHQCDRGWA PVFLDREQSI SLMSVGFLLE KPDEAVVWRG PKKNALIKQF VSDVAWGELD YLVVDTPPGT SDEHMATIEA LRPYQPLGAL VVTTPQAVSV GDVRRELTFC RKTGLRVMGI VENMSGFTCP HCTECTSVFS RGGGEELAQL AGVPFLGSVP LDPALMRTLE EGHDFIQEFP GSPAFAALTS IAQKILDATP ACLP