Recombinant humaan NUDT14/UGPP-eiwit

Uridine diphosphate glucose pyrophosphatase, also known as NuDT14, is a 222 amino acid cytoplasmic protein that contains one nudix hydrolase domain and belongs to the nudix hydrolase family. NuDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and uDP-glucose to glucose 1-phosphate and uMP. Existing as a homodimer, NuDT14 binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14. Recombinant human NuDT14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nudix hydrolase 14, Uridine diphosphate glucose pyrophosphatase NUDT14, UDPG pyrophosphatase, UGPPase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.5 kDa (245aa) confirmed by MALDI-TOF

NUDT14, uridine diphosphate glucose pyrophosphatase, uGPP, uGPPase, ATGP1965-10 µg, ATGP1965-20 µg, ATGP1965-50 µg, ATGP1965-100 µg, ATGP1965-250 µg, ATGP1965-500 µg, ATGP1965-1 mg, ATGP1965-10, ATGP1965-20, ATGP1965-50, ATGP1965-100, ATGP1965-250, ATGP1965-500, ATGP1965-1

Yagi T., et al. (2003) Biochem J. 370:409-415.; ; Heyen C A., et al. (2009) Biochem Biophys Res Commun. 390: 1414-1418.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_803877.2

O95848

Human

MERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ