Recombinant humaan NUDT16-eiwit

NuDT16 is nuclear nucleoside diphosphatase proteins localized within foci in the nucleolus and nucleoplasm. This is a RNA-decapping enzyme that binds specifically to u8 snoRNA. It can remove m7G and m227G caps from RNAs, rendering them substrates for 5'-3' exonucleases for degradation in vivo. Recombinant human NuDT16 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nudix type motif 16, Nudix hydrolase 16, U8 snoRNA-decapping enzyme, IDP phosphatase, IDPase, Nosine diphosphate phosphatase, Nucleoside diphosphate-linked moiety X motif 16, U8 snoRNA-binding protein H29K, m7GpppN-mRNA hydrolase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.4 kDa (215aa) confirmed by MALDI-TOF

NUDT16, Nudix type motif 16, ATGP0868-10 µg, ATGP0868-20 µg, ATGP0868-50 µg, ATGP0868-100 µg, ATGP0868-250 µg, ATGP0868-500 µg, ATGP0868-1 mg, ATGP0868-10, ATGP0868-20, ATGP0868-50, ATGP0868-100, ATGP0868-250, ATGP0868-500, ATGP0868-1

Peculis B.A., et al. (2007) J. Biol. Chem. 282:24792-24805.; ; Ghosh T., et al. (2004) Mol. Cell 13:817-828.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Enzymes & Proteases

NP_689608

Q96DE0

Human

MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH