Recombinant humaan NUDT1/MTH1-eiwit

NuDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. It is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. And this protein expressed at much higher levels in proliferating cells than in resting cells. Recombinant human NuDT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Oxidized purine nucleoside triphosphate hydrolase, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, Methylated purine nucleoside triphosphate hydrolase, Nucleoside diphosphate-linked moiety X motif 1 (Nudix motif 1)

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

20.1 kDa (176aa) confirmed by MALDI-TOF

"7,8-dihydro-8-oxoguanine triphosphatase", 78-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21, MTH1, Nudix motif 1, NUDT1, ATGP0742-10 µg, ATGP0742-20 µg, ATGP0742-50 µg, ATGP0742-100 µg, ATGP0742-250 µg, ATGP0742-500 µg, ATGP0742-1 mg, ATGP0742-010, ATGP0742-020, ATGP0742-050, ATGP0742-100, ATGP0742-250, ATGP0742-500, ATGP0742-01M

Satoh J., et al (2000) Eur J Neurol. 7 (6) :673-7.; ; Oda H., et al. (1997) J. Biol. Chem. 272:17843-17850;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl

Enzymes & Proteases

NP_945191

P36639

Human

MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV