Recombinant humaan NUDT2/APAH1-eiwit

NuDT2 is a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NuDIX hydrolase family. This protein maintains homeostasis by using water to cleave the metabolite NuDT symmetrically back into its original ATP and AMP molecules. NuDT2 is also active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, but not towards diadenosine triphosphate. NuDT2 is involved in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations. Recombinant NuDT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nudix hydrolase 2, Bis (5'-nucleosyl) -tetraphosphatase asymmetrical, nudix (nucleoside diphosphate linked moiety X) -type motif 2, Ap4A hydrolase 1, Ap4Aase, diadenosine tetraphosphatase, diadenosine 5',5''-P1, P4-tetraphosphate pyrophosphohydrolase, APAH1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19 kDa (167aa) confirmed by MALDI-TOF

NUDT2, bis (5'-nucleosyl) -tetraphosphatase [asymmetrical], APAH1, MGC10404, ATGP0890-10 µg, ATGP0890-20 µg, ATGP0890-50 µg, ATGP0890-100 µg, ATGP0890-250 µg, ATGP0890-500 µg, ATGP0890-1 mg, ATGP0890-10, ATGP0890-20, ATGP0890-50, ATGP0890-100, ATGP0890-250, ATGP0890-500, ATGP0890-1

Swarbrick JD., et al. (2005) J Biol Chem. 280 (9) :8471-81.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Enzymes & Proteases

NP_671702

P50583

Human

MGSSHHHHHH SSGLVPRGSH MALRACGLII FRRCLIPKVD NNAIEFLLLQ ASDGIHHWTP PKGHVEPGED DLETALRETQ EEAGIEAGQL TIIEGFKREL NYVARNKPKT VIYWLAEVKD YDVEIRLSHE HQAYRWLGLE EACQLAQFKE MKAALQEGHQ FLCSIEA