Recombinant humaan NUDT3/DIPP1-eiwit

NuDT3, also known as DIPP, DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), is a 172 amino acid cytoplasmic protein belonging to the nudix hydrolase family and DIPP subfamily. NuDT3 acts as a negative regulator of the ERK 1/2 pathway and hydrolyzes 5-phosphoribose 1-diphosphate. NuDT3 exists as a monomer and binds magnesium as a cofactor. Also NuDT3 is widely expressed but found at highest levels in liver, pancreas, brain and heart. Recombinant human NuDT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nudix hydrolase 3, Diadenosine 5',5'''-P1, P6-hexaphosphate hydrolase 1, Diphosphoinositol polyphosphate phosphohydrolase 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

21.6 kDa (192aa) confirmed by MALDI-TOF

NUDT3, Nudix (nucleoside diphosphate linked moiety X) -type motif 3, DIPP, DIPP1, ATGP0922-10 µg, ATGP0922-20 µg, ATGP0922-50 µg, ATGP0922-100 µg, ATGP0922-250 µg, ATGP0922-500 µg, ATGP0922-1 mg, ATGP0922-10, ATGP0922-20, ATGP0922-50, ATGP0922-100, ATGP0922-250, ATGP0922-500, ATGP0922-1

Chu C., et al. (2004) Cell Signal. 16 (9) :1045-59.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Signal Transduction

NP_006694

O95989

Human

MGSSHHHHHH SSGLVPRGSH MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ YHKPVQASYF ETLRQGYSAN NGTPVVATTY SVSAQSSMSG IR