Recombinant humaan O-fucosyltransferase 1/POFUT1-eiwit

GDP-fucose protein O-fucosyltransferase 1, also known as POuFuT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFuT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFuT1 plays an important role in Notch signaling, as Notch ligands can serve as POFuT1 substrates. Recombinant human POFuT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

GDP-fucose protein O-fucosyltransferase 1, FuT12, O-Fuc-T, O-FucT-1, O-FuT

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

43.7 kDa (385aa)

O-fucosyltransferase1, POFUT1, GDP-fucose protein O-fucosyltransferase 1, FuT12, O-Fuc-T, O-FucT-1, O-FuT, ATGP1913-10 µg, ATGP1913-20 µg, ATGP1913-50 µg, ATGP1913-100 µg, ATGP1913-250 µg, ATGP1913-500 µg, ATGP1913-1 mg, ATGP1913-10, ATGP1913-20, ATGP1913-50, ATGP1913-100, ATGP1913-250, ATGP1913-500, ATGP1913-1

Wang Y., et al. (2001) J Biol Chem. 276:40338-40345.; ; Luo Y., et al. (2005) J Biol Chem. 280:11289-11294.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol

Neuroscience

NP_056167

Q9H488

Human

GSWDPAG YLLYCPCMGR FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL EPLQAYHRVI SLEDFMEKLA PTHWPPEKRV AYCFEVAAQR SPDKKTCPMK EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YREQWSQRFS PKEHPVLALP GAPAQFPVLE EHRPLQKYMV WSDEMVKTGE AQIHAHLVRP YVGIHLRIGS DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TAAPLTMTMC LPDLKEIQRA VKLWVRSLDA QSVYVATDSE SYVPELQQLF KGKVKVVSLK PEVAQVDLYI LGQADHFIGN CVSSFTAFVK RERDLQGRPS SFFGMDRPPK LRDEF