Recombinant humaan OAZ1-eiwit

Ornithine decarboxylase antizyme 1, also known as OAZ1, belongs to the ODC antizyme family. It expression is auto-regulated by polyamine-enhanced translational frameshifting. OAZ1 negatively regulates polyamine synthesis by enhancing the negative feedback loop controlling ornithine decarboxylase (ODC) activity. The OAZ1 encoded by this gene inhibits ornithine decarboxylase and accelerates its degradation. Recombinant human OAZ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ornithine decarboxylase antizyme 1, AZI, OAZ

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.8 kDa (251aa) confirmed by MALDI-TOF

OAZ1, Ornithine decarboxylase antizyme 1, AZI, OAZ, ATGP1694-10 µg, ATGP1694-20 µg, ATGP1694-50 µg, ATGP1694-100 µg, ATGP1694-250 µg, ATGP1694-500 µg, ATGP1694-1 mg, ATGP1694-10, ATGP1694-20, ATGP1694-50, ATGP1694-100, ATGP1694-250, ATGP1694-500, ATGP1694-1

Zhang M., et al. (2003) EMBO J. 22: 1488-1496; ; Aoto H., et al. (1997) Genomics. 40: 138-141.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol,1mM DTT

Metabolism

NP_004143.1

P54368

Human

MVKSSLQ RILNSHCFAR EKEGDKPSAT IHASRTMPLL SLHSRGGSSS ESSRVSLHCC SNPGPGPRWC SDAPHPPLKI PGGRGNSQRD HNLSANLFYS DDRLNVTEEL TSNDKTRILN VQSRLTDAKR INWRTVLSGG SLYIEIPGGA LPEGSKDSFA VLLEFAEEQL RADHVFICFH KNREDRAALL RTFSFLGFEI VRPGHPLVPK RPDACFMAYT FERESSGEEE E