Recombinant humaan ODC1-eiwit

Ornithine decarboxylase, also known as ODC1, is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. ODC1 plays an important role in diverse biological processes, including cell growth, differentiation, transformation and apoptosis. Overexpression of the ODC1 gene plays important roles in cell proliferation and the development of cancer. Recombinant human ODC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ornithine decarboxylase, ODC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

53.5 kDa (484aa)

ODC1, Ornithine decarboxylase, ODC, ATGP1253-10 µg, ATGP1253-20 µg, ATGP1253-50 µg, ATGP1253-100 µg, ATGP1253-250 µg, ATGP1253-500 µg, ATGP1253-1 mg, ATGP1253-10, ATGP1253-20, ATGP1253-50, ATGP1253-100, ATGP1253-250, ATGP1253-500, ATGP1253-1

Moshier J A., et al. (1992) Genomics. 1:77-86.; ; Yang Feng T L., et al. (1996) Genomics. 36:449-458.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_002530

P11926

Human

MNNFGNE EFDCHFLDEG FTAKDILDQK INEVSSSDDK DAFYVADLGD ILKKHLRWLK ALPRVTPFYA VKCNDSKAIV KTLAATGTGF DCASKTEIQL VQSLGVPPER IIYANPCKQV SQIKYAANNG VQMMTFDSEV ELMKVARAHP KAKLVLRIAT DDSKAVCRLS VKFGATLRTS RLLLERAKEL NIDVVGVSFH VGSGCTDPET FVQAISDARC VFDMGAEVGF SMYLLDIGGG FPGSEDVKLK FEEITGVINP ALDKYFPSDS GVRIIAEPGR YYVASAFTLA VNIIAKKIVL KEQTGSDDED ESSEQTFMYY VNDGVYGSFN CILYDHAHVK PLLQKRPKPD EKYYSSSIWG PTCDGLDRIV ERCDLPEMHV GDWMLFENMG AYTVAAASTF NGFQRPTIYY VMSGPAWQLM QQFQNPDFPP EVEEQDASTL PVSCAWESGM KRHRAACASA SINV