Recombinant humaan OGG1-eiwit

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS) . It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

8-oxoguanine DNA glycosylase, N-glycosylase/DNA lyase, DNA-apurinic or apyrimidinic site lyase, AP lyase, HMMH, HOGG1, OGH1, MUTM

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.2 kDa (368aa) confirmed by MALDI-TOF

8-oxoGuanine DNA Glycosylase, OGG1, 8-oxoguanine DNA glycosylase 1, HMMH, HOGG1, MuTM, OGH1, AP lyase, 8-oxoguanine DNA glycosylase 1, ATGP0668-10 µg, ATGP0668-20 µg, ATGP0668-50 µg, ATGP0668-100 µg, ATGP0668-250 µg, ATGP0668-500 µg, ATGP0668-1 mg, ATGP0668-10, ATGP0668-20, ATGP0668-50, ATGP0668-100, ATGP0668-250, ATGP0668-500, ATGP0668-1

Seeberg E., et al. (2002) . Nucleic Acids Res. 30 (11) :2349-57.; ; Hodges NJ., et al (2010) . DNA Repair (Amst) . 9 (2) :144-52.;

Duan S, et al. Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase. (Nucleic Acids Res. 2020) {https://pubmed.ncbi.nlm.nih.gov/32432680/}; ; Schniertshauer D, et al. Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts. (Bio Protoc. 2021) {https://pubmed.ncbi.nlm.nih.gov/33855116/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Enzymes & Proteases

AAH00657.1

O15527

Human

MPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG